Journal: Immunity, Inflammation and Disease

Article Title: Data mining combined with experiments to validate CEP55 as a prognostic biomarker in colorectal cancer

doi: 10.1002/iid3.375

Figure Lengend Snippet: Screening differential expression genes (DEGs) and enrichment analysis of Hub genes. (A) Venn diagram. DEGs were selected with a |log2 (fold change)| > 1 and adjust p < .01 among the messenger RNA (mRNA) expression profiling sets GSE110225, GSE22598, and GSE37364. The three datasets showed an overlap of 284 genes. (B) Volcano graph. Differential analysis was performed on 437 colorectal cancer (CRC) samples (398 tumor tissues and 39 paired tissues adjacent to cancer) in The Cancer Genome Atlas Program (TCGA), and 6500 differentially expressed genes were screened (4477 upregulated and 2023 downregulated). (C) The top 12 GO and pathway cluster networks obtained by enrichment analysis of the 28 selected target genes

Article Snippet: The elevated CEP55 messenger RNA (mRNA) was observed in CRC tissues using RT‐PCR in an OriGene Colon Cancer cDNA array (Figure ).

Techniques: Expressing

Journal: Immunity, Inflammation and Disease

Article Title: Data mining combined with experiments to validate CEP55 as a prognostic biomarker in colorectal cancer

doi: 10.1002/iid3.375

Figure Lengend Snippet: Functional roles of 28 hub genes

Article Snippet: The elevated CEP55 messenger RNA (mRNA) was observed in CRC tissues using RT‐PCR in an OriGene Colon Cancer cDNA array (Figure ).

Techniques: Functional Assay, Expressing, Activation Assay, DNA Synthesis, Transduction, Variant Assay, Over Expression, Migration, Transformation Assay, Binding Assay, Sequencing, Marker, Amplification

Journal: Immunity, Inflammation and Disease

Article Title: Data mining combined with experiments to validate CEP55 as a prognostic biomarker in colorectal cancer

doi: 10.1002/iid3.375

Figure Lengend Snippet: CEP55 is highly expressed in CRC and is associated with multiple clinical factors. (A,B) CEP55 expression in multiple diseases and the expression levels of CEP55 in four CRC datasets (Oncomine). (C) The expression difference of CEP55 in CRC tumor tissue and matching adjacent cancer tissues (sample data from TCGA). (D,E) Expression of CEP55 in extensive sample data and human organs (GEPIA2). (F) The expression of CEP55 was correlated with cancer stage, patient's age, and nodal metastasis status under TCGA samples (UALCAN). CRC, colorectal cancer; TCGA, The Cancer Genome Atlas Program

Article Snippet: The elevated CEP55 messenger RNA (mRNA) was observed in CRC tissues using RT‐PCR in an OriGene Colon Cancer cDNA array (Figure ).

Techniques: Expressing

Journal: Immunity, Inflammation and Disease

Article Title: Data mining combined with experiments to validate CEP55 as a prognostic biomarker in colorectal cancer

doi: 10.1002/iid3.375

Figure Lengend Snippet: CEP55 is unregulated in human CRC tissues. (A) The DNA expression level of CEP55 has analyzed agarose gel electrophoresis assays. (B) CEP55 mRNA expression was analyzed in CRC samples and the corresponding para cancer tissue samples. (C) Representative images of HE staining and CEP55 staining (brown color) in CRC samples (T) and normal colon tissue (N) (scale bar = 50 μm). (D) IHC scores of tumors and adjacent normal tissues from 37 paired CRC specimens. (E,F) CEP55 protein expression was analyzed by Western blot in 24 human CRC tissues (T) and the corresponding para cancer tissue samples (N) by Western blot analysis. (G,H) Western blot analysis of CEP55 from normal colon cell line (NCM460) and five CRC cell lines. CRC, colorectal cancer; HE, hematoxylin and eosin; IHC, mmunohistochemistry; mRNA, messenger RNA. * p < .05, ** p < .01, *** p < .001

Article Snippet: The elevated CEP55 messenger RNA (mRNA) was observed in CRC tissues using RT‐PCR in an OriGene Colon Cancer cDNA array (Figure ).

Techniques: Expressing, Agarose Gel Electrophoresis, Staining, Western Blot

Journal: Immunity, Inflammation and Disease

Article Title: Data mining combined with experiments to validate CEP55 as a prognostic biomarker in colorectal cancer

doi: 10.1002/iid3.375

Figure Lengend Snippet: CEP55 promotes the proliferation of CRC cells in vitro by the p53 signaling pathway. (A) Cell proliferation assay was performed by Edu proliferation assays. (B) Colony formation assay after 14 days of culture, with a mean colony counts from three independent experiments. (C) CEP55 regulated the expression of the p53/p21 signaling proteins. Knockdown of CEP55 activated p53/p21 signaling pathway in SW480 and Caco‐2 cells. The protein level of CEP55, p53, and p21 were detected and quantified in CEP55 Knockdowning SW480 and Caco‐2 cells; GAPDH was used as a loading control. CRC, colorectal cancer; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase. * p < .05, ** p < .01, *** p < .001

Article Snippet: The elevated CEP55 messenger RNA (mRNA) was observed in CRC tissues using RT‐PCR in an OriGene Colon Cancer cDNA array (Figure ).

Techniques: In Vitro, Proliferation Assay, Colony Assay, Expressing